4PPI
Crystal structure of Bcl-xL hexamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 153.630, 153.630, 153.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.158 - 2.851 |
| R-factor | 0.2396 |
| Rwork | 0.236 |
| R-free | 0.26870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b48 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.982 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.350 | 3.010 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.086 | 0.702 |
| Number of reflections | 7536 | |
| <I/σ(I)> | 28.3 | 5.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 38.8 | 41.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 1.0M sodium citrate, 0.1M CHES pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
