4PMW
Structure of mouse Dis3L2 in complex with oligoU RNA substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-11 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.927, 96.075, 157.311 |
Unit cell angles | 90.00, 98.75, 90.00 |
Refinement procedure
Resolution | 63.182 - 2.950 |
R-factor | 0.2046 |
Rwork | 0.202 |
R-free | 0.25120 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 0.957 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 96.100 | 96.070 | 3.300 |
High resolution limit [Å] | 2.950 | 6.600 | 2.950 |
Rmerge | 0.126 | 0.063 | 0.463 |
Rpim | 0.080 | 0.040 | 0.290 |
Total number of observations | 136961 | 12265 | 39093 |
Number of reflections | 39795 | ||
<I/σ(I)> | 8 | 16.3 | 2.9 |
Completeness [%] | 99.9 | 99.6 | 99.9 |
Redundancy | 3.4 | 3.4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 100 mM ammonium citrate pH 5.5, 200 mM ammonium chloride, 22 % PEG 3350 |