4PMI

Crystal structure of Rev and Rev-response-element RNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.115869
Spacegroup name	P 41 3 2
Unit cell lengths	165.300, 165.300, 165.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.846 - 3.200
R-factor	0.1946
Rwork	0.193
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lph 1etf
RMSD bond length	0.002
RMSD bond angle	0.394
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.850	45.850	3.310
High resolution limit [Å]	3.200	14.310	3.200
Rmerge	0.115	0.038
Rmeas	0.117	0.039	2.089
Total number of observations	343444
Number of reflections	13246	268	1751
<I/σ(I)>	23.47	78.14	1.74
Completeness [%]	99.7	97.1	98.36
Redundancy	25.9		15.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	292	50 mM MES pH 6.0, 50 mM KCl, 1-4% PEG 4000,