4PM5
Crystal structure of CTX-M-14 S70G beta-lactamase in complex with cefotaxime at 1.26 Angstroms resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2014-04-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.550, 62.680, 86.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.451 - 1.261 |
R-factor | 0.1627 |
Rwork | 0.162 |
R-free | 0.18220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ylt |
RMSD bond length | 0.005 |
RMSD bond angle | 1.132 |
Data reduction software | iMOSFLM (1.0.7) |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.500 |
High resolution limit [Å] | 1.260 |
Number of reflections | 61202 |
<I/σ(I)> | 19.1 |
Completeness [%] | 99.7 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296 | Ammonium phosphate, PEG 4000 |