4PLW
Crystal structure of ancestral apicomplexan malate dehydrogenase with lactate.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2012-09-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.115866 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 90.367, 103.738, 143.723 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.789 - 1.850 |
R-factor | 0.1828 |
Rwork | 0.182 |
R-free | 0.22120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hjr |
RMSD bond length | 0.005 |
RMSD bond angle | 1.012 |
Data reduction software | XDS |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.960 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.148 | 3.350 |
Number of reflections | 115626 | |
<I/σ(I)> | 12.47 | 0.66 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 8.1 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20mg/mL protein with 2mM NADH/L-lactate; well solution: 20% (w/v) PEG-1500, 0.1M HEPES, pH7.5 |