4PL8

Structure of rabbit skeletal muscle actin in complex with a hybrid peptide comprising thymosin beta4 and the lysine-rich region of Cordon-Bleu

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 43 21 2
Unit cell lengths	93.640, 93.640, 206.274
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.896 - 2.000
R-factor	0.1728
Rwork	0.171
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t44
RMSD bond length	0.008
RMSD bond angle	1.163
Data reduction software	SCALEPACK
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.070
High resolution limit [Å]	2.000	4.300	2.000
Rmerge	0.060	0.028	0.595
Total number of observations	751602
Number of reflections	62837
<I/σ(I)>	12.5
Completeness [%]	100.0	100	100
Redundancy	12	11.8	11.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.5	288	0.1 M citric acid, 15% (w/v) PEG 3350