4PL8
Structure of rabbit skeletal muscle actin in complex with a hybrid peptide comprising thymosin beta4 and the lysine-rich region of Cordon-Bleu
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 93.640, 93.640, 206.274 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.896 - 2.000 |
| R-factor | 0.1728 |
| Rwork | 0.171 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t44 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.163 |
| Data reduction software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.300 | 2.000 |
| Rmerge | 0.060 | 0.028 | 0.595 |
| Total number of observations | 751602 | ||
| Number of reflections | 62837 | ||
| <I/σ(I)> | 12.5 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 12 | 11.8 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 288 | 0.1 M citric acid, 15% (w/v) PEG 3350 |
