4PJ6
Crystal Structure of Human Insulin Regulated Aminopeptidase with Lysine in Active Site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.031, 256.621, 71.310 |
Unit cell angles | 90.00, 114.35, 90.00 |
Refinement procedure
Resolution | 48.320 - 2.960 |
R-factor | 0.18899 |
Rwork | 0.185 |
R-free | 0.25669 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.580 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.320 | 3.060 |
High resolution limit [Å] | 2.960 | 2.960 |
Number of reflections | 46240 | |
<I/σ(I)> | 12.4 | 1.7 |
Completeness [%] | 99.6 | 98.8 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 3000, tris hydrochloride |