4PHH
Crystal structure of Ypt7 covalently modified with GNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.92045 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.570, 72.230, 82.340 |
Unit cell angles | 90.00, 91.49, 90.00 |
Refinement procedure
Resolution | 48.180 - 2.350 |
R-factor | 0.20331 |
Rwork | 0.200 |
R-free | 0.26301 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.672 |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.180 | 2.450 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 29719 | |
<I/σ(I)> | 18.28 | |
Completeness [%] | 99.9 | |
Redundancy | 6.88 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M Tris pH 7, 25 % PEG 6000, 1 M LiCl |