4PF7
Crystal structure of insulin degrading enzyme complexed with inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-09 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97931 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 78.839, 115.961, 124.282 |
Unit cell angles | 90.00, 97.83, 90.00 |
Refinement procedure
Resolution | 19.940 - 2.330 |
R-factor | 0.1993 |
Rwork | 0.199 |
R-free | 0.23560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g47 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.070 |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.390 |
High resolution limit [Å] | 2.330 | 2.330 |
Rmerge | 0.650 | |
Number of reflections | 98275 | |
<I/σ(I)> | 5.5 | 2.2 |
Completeness [%] | 99.4 | 98.7 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | 20% PEG3350, 0.2 mM sodium thiocyanate |