4PDQ
Crystal structure of the bacterial ribosomal decoding site in complex with 4'-deoxy-4'-fluoro neomycin analog
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-18 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98 |
Spacegroup name | H 3 |
Unit cell lengths | 103.657, 103.657, 44.572 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.600 - 3.000 |
R-factor | 0.195 |
Rwork | 0.195 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | PHENIX |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.600 |
High resolution limit [Å] | 3.000 |
Number of reflections | 3522 |
<I/σ(I)> | 12.6 |
Completeness [%] | 98.7 |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Sodium cacodylate, MgCl2, MPD, Spermine tetrahydrochloride |