4PDK
FadR, Fatty Acid Responsive Transcription Factor from Vibrio cholerae, in Complex with oleoyl-CoA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2013-09-28 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 116.920, 88.690, 62.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.730 - 2.800 |
R-factor | 0.2628 |
Rwork | 0.259 |
R-free | 0.29820 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 2.148 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER ((phenix.molecular replacement: 1.8.4_1496)) |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.730 |
High resolution limit [Å] | 2.800 |
Number of reflections | 16577 |
<I/σ(I)> | 11.48 |
Completeness [%] | 99.3 |
Redundancy | 3.63 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 17% PEG 3350, 0.23M magnesium formate |