4PDI
Crystal structure of a complex between an inhibited LlFpg and a N7-Benzyl-Fapy-dG containing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-09-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.54980 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 91.535, 91.535, 140.974 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.670 - 2.100 |
| R-factor | 0.1616 |
| Rwork | 0.160 |
| R-free | 0.19020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c58 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.075 |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.670 | 2.220 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.103 | 0.705 |
| Number of reflections | 66600 | |
| <I/σ(I)> | 17 | 3 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 13.7 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | HEPES, SODIUM CITRATE |
