4PC3

Elongation factor Tu:Ts complex with partially bound GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	CCD
Collection date	2003-12-03
Detector	MAR CCD 165 mm
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	74.000, 108.920, 194.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.920 - 1.831
R-factor	0.1611
Rwork	0.159
R-free	0.19850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pc1
RMSD bond length	0.012
RMSD bond angle	1.276
Refinement software	PHENIX ((phenix.refine: dev_1702))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.920	1.950
High resolution limit [Å]	1.830	1.830
Rmerge	0.052	0.369
Number of reflections	136637
<I/σ(I)>	16.3	3.9
Completeness [%]	98.5	98.2
Redundancy	6.5	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	100 mM MES, 15% PEG 6000, 2.5% glucose, 76mM NH4 CH3COO, 100mM NH4-citrate, 5mM MgCl2, 1mM GDP