4PBP
crystal structure of zebrafish short-chain pentraxin protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 65 |
| Unit cell lengths | 153.623, 153.623, 62.330 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.137 - 1.648 |
| R-factor | 0.1539 |
| Rwork | 0.153 |
| R-free | 0.17100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pbo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.395 |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.648 |
| Number of reflections | 100591 |
| <I/σ(I)> | 18.836 |
| Completeness [%] | 99.4 |
| Redundancy | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.085M HEPES sodium, 8.5% v/v 2-Propanol, 17% w/v Polyethylene glycol 4000, 15% (v/v) Glycerol |
