4P9Z
Grb2 SH2 complexed with a pTyr-Ac6c-Asn tripeptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-12-10 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 41.905, 41.905, 107.642 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.800 |
R-factor | 0.18367 |
Rwork | 0.181 |
R-free | 0.23943 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.889 |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.087 | 0.331 |
Number of reflections | 9395 | |
<I/σ(I)> | 19 | 8.8 |
Completeness [%] | 98.3 | 100 |
Redundancy | 7.1 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296 | 0.2 M sodium acetate trihydrate, 0.1 M TRIS hydrochloride, 30% w/v polyethylene glycol 4000 |