4P9M
Crystal structure of 8ANC195 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-11 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 66.482, 66.482, 219.034 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.530 - 2.130 |
R-factor | 0.212 |
Rwork | 0.211 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u7w |
RMSD bond length | 0.010 |
RMSD bond angle | 1.296 |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.534 | ||
High resolution limit [Å] | 1.980 | 6.270 | 2.090 |
Rmerge | 0.112 | ||
Rmeas | 0.142 | ||
Rpim | 0.049 | ||
Number of reflections | 28097 | ||
<I/σ(I)> | 11.9 | ||
Completeness [%] | 98.9 | 99.1 | 100 |
Redundancy | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 298 | PEG 6000, HEPES, zinc chloride |