4P6H
Tl+-bound inward-facing state (bound conformation) of the glutamate transporter homologue GltPh
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-01-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 110.830, 200.434, 206.397 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 4.080 |
R-factor | 0.26214 |
Rwork | 0.258 |
R-free | 0.29620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kbc |
RMSD bond length | 0.012 |
RMSD bond angle | 1.407 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 4.140 |
High resolution limit [Å] | 4.000 | 4.000 |
Rmerge | 0.752 | |
Number of reflections | 23932 | |
<I/σ(I)> | 7.9 | 1.3 |
Completeness [%] | 65.3 | |
Redundancy | 3.4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 400, Potassium Citrate, Potassium Chloride, Magnesium Chloride |