4P6H
Tl+-bound inward-facing state (bound conformation) of the glutamate transporter homologue GltPh
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 110.830, 200.434, 206.397 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 4.080 |
| R-factor | 0.26214 |
| Rwork | 0.258 |
| R-free | 0.29620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kbc |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.407 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 4.140 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.752 | |
| Number of reflections | 23932 | |
| <I/σ(I)> | 7.9 | 1.3 |
| Completeness [%] | 65.3 | |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 400, Potassium Citrate, Potassium Chloride, Magnesium Chloride |
