4P65
Crystal structure of an cyclohexylalanine substituted insulin analog.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.040, 60.900, 59.300 |
| Unit cell angles | 90.00, 112.25, 90.00 |
Refinement procedure
| Resolution | 42.300 - 1.500 |
| R-factor | 0.1641 |
| Rwork | 0.160 |
| R-free | 0.20260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1znj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.351 |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.300 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.840 | |
| Number of reflections | 54128 | |
| <I/σ(I)> | 13.9 | 1.6 |
| Completeness [%] | 95.8 | 99.3 |
| Redundancy | 1.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | sodium citrate, phenol, sodium chloride, zinc acetate, tris |
