4P5X
Structure of the N-terminal domain of the human mitochondrial aspartate/glutamate carrier Aralar in the calcium-bound state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-08-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.898 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 54.450, 54.450, 168.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.030 - 2.261 |
R-factor | 0.2334 |
Rwork | 0.231 |
R-free | 0.27410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4p5w |
RMSD bond length | 0.005 |
RMSD bond angle | 0.543 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.033 | 2.340 |
High resolution limit [Å] | 2.260 | 2.260 |
Rmerge | 0.044 | 0.859 |
Number of reflections | 14197 | |
<I/σ(I)> | 21.3 | 2.2 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 6.4 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 0.1M MIB, 25% PEG 1500 |