4P5K
Structural Basis of Chronic Beryllium Disease: Bridging the Gap Between Allergy and Autoimmunity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.99 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 182.779, 68.738, 71.133 |
Unit cell angles | 90.00, 105.72, 90.00 |
Refinement procedure
Resolution | 44.620 - 2.590 |
R-factor | 0.2245 |
Rwork | 0.221 |
R-free | 0.28100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.626 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.600 |
Number of reflections | 26282 |
<I/σ(I)> | 18.6 |
Completeness [%] | 92.6 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 316 | 0.2 M ammonium formate and 25 % PEG 4000 in 0.1 M HEPES buffer |