4P4O

Crystal structure of Leishmania infantum polymerase beta: Ternary gap complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-04-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1
Spacegroup name	P 65 2 2
Unit cell lengths	108.680, 108.680, 151.641
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.060 - 2.300
R-factor	0.197
Rwork	0.194
R-free	0.22800
RMSD bond length	0.008
RMSD bond angle	1.125
Refinement software	PHENIX ((PHENIX.REFINE: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	94.130
High resolution limit [Å]	2.300
Number of reflections	24191
<I/σ(I)>	31.4
Completeness [%]	100.0
Redundancy	21.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M magnesium formate