4P2O
Crystal structure of the 2B4 TCR in complex with 2A/I-Ek
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 239.936, 60.176, 78.359 |
| Unit cell angles | 90.00, 104.33, 90.00 |
Refinement procedure
| Resolution | 47.667 - 2.603 |
| R-factor | 0.1916 |
| Rwork | 0.189 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.695 |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.428 | |
| Number of reflections | 32517 | |
| <I/σ(I)> | 13.6 | 2 |
| Completeness [%] | 96.9 | 88.6 |
| Redundancy | 3.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 18-20% PEG 3350, 0.02M Na/K Phosphate, 0.1M Bis Tris Propane pH 8.5 |
