4P18
Crystal Structure of frog M ferritin mutant D80K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.980 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 238.930, 238.430, 119.690 |
Unit cell angles | 90.00, 94.33, 90.00 |
Refinement procedure
Resolution | 75.350 - 1.910 |
R-factor | 0.19791 |
Rwork | 0.196 |
R-free | 0.23082 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lqh |
RMSD bond length | 0.010 |
RMSD bond angle | 1.253 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.380 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.081 | 0.149 |
Number of reflections | 508671 | |
<I/σ(I)> | 9.4 | 5.3 |
Completeness [%] | 97.2 | 91.9 |
Redundancy | 3.4 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | 1.0 M Ammonium sulfate, 0.1 M Sodium acetate pH 4.6 |