4P0S
human Mus81-Eme1-3'flap DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2013-09-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 92.442, 250.758, 430.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.978 - 6.000 |
R-factor | 0.2533 |
Rwork | 0.250 |
R-free | 0.30790 |
RMSD bond length | 0.018 |
RMSD bond angle | 2.353 |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 5.920 |
Number of reflections | 16874 |
<I/σ(I)> | 43.7 |
Completeness [%] | 99.5 |
Redundancy | 9.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG400, Tris, DTT |