4P0A
Crystal structure of HOIP PUB domain in complex with p97 PIM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2013-04-17 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 |
Unit cell lengths | 48.040, 48.040, 266.622 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.604 - 2.300 |
R-factor | 0.2018 |
Rwork | 0.199 |
R-free | 0.24870 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.192 |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 53.320 |
High resolution limit [Å] | 2.300 |
Number of reflections | 15446 |
<I/σ(I)> | 14.9 |
Completeness [%] | 99.9 |
Redundancy | 9.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0,1M Tris, 20% 2-methyl-2,4-pentanediol, 15% Polyethyleneglycol 3350, pH 6.5 |