4OYN

Fifteen minutes iron loaded human H ferritin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-05
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.920
Spacegroup name	F 4 3 2
Unit cell lengths	183.910, 183.910, 183.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.090 - 1.430
R-factor	0.16757
Rwork	0.167
R-free	0.17948
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ajo
RMSD bond length	0.005
RMSD bond angle	0.981
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.510	1.510
High resolution limit [Å]	1.430	1.430
Rmerge	0.068	0.417
Number of reflections	49578
<I/σ(I)>	27.9	7.1
Completeness [%]	100.0	100
Redundancy	20	20.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	277	1.6 M MgCl2 and 0.1 M bicine pH 9.0