4OYJ
Structure of the apo HOIP PUB domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-10 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 155.190, 99.540, 173.730 |
Unit cell angles | 90.00, 99.90, 90.00 |
Refinement procedure
Resolution | 62.605 - 3.000 |
R-factor | 0.2094 |
Rwork | 0.207 |
R-free | 0.25450 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.753 |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.750 | 3.090 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.126 | 0.451 |
Number of reflections | 51318 | |
<I/σ(I)> | 6.9 | 2.3 |
Completeness [%] | 98.1 | 98.7 |
Redundancy | 2.8 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 1.3 M ammonium sulphate, 200 mM KI, 100 mM Tris, pH 8.5 |