4OWZ
Structure of ECP/H15A mutant.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 62.543, 62.543, 175.233 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.452 - 1.470 |
R-factor | 0.2067 |
Rwork | 0.206 |
R-free | 0.22330 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.147 |
Refinement software | PHENIX ((PHENIX.REFINE: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 175.230 | 1.580 |
High resolution limit [Å] | 1.470 | 1.470 |
Rmerge | 0.042 | 0.447 |
Number of reflections | 60201 | |
<I/σ(I)> | 34.9 | 6.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 15.9 | 14.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | Protein was resuspended in 20mM sodium acetate buffer, pH 5.0, 1 uL of sample mixed with 1 uL of crystallisation buffer (0.1M sodium citrate, pH 5.2; 8% Jeffamine M-600; 10mM iron(III) chloride) |