4OWY
Crystal Structure of Ahp1 from Saccharomyces cerevisiae. Investigating the electron transfers.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2007-04-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.421 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.127, 127.799, 66.421 |
| Unit cell angles | 90.00, 103.24, 90.00 |
Refinement procedure
| Resolution | 42.600 - 2.200 |
| R-factor | 0.184 |
| Rwork | 0.180 |
| R-free | 0.24200 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.858 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.640 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.069 | 0.416 |
| Number of reflections | 32233 | |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289.15 | 20% PEG 3000, 0.2M CaAc and 0.1M Bis-Tris Propane |
