4OWF
Crystal structure of the NEMO CoZi in complex with HOIP NZF1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.28254 |
| Spacegroup name | P 65 |
| Unit cell lengths | 81.410, 81.410, 74.501 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.960 - 2.000 |
| R-factor | 0.26152 |
| Rwork | 0.259 |
| R-free | 0.31267 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | . 3FX0 AND 2WX0 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.369 |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.960 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.136 | 1.107 |
| Number of reflections | 15849 | |
| <I/σ(I)> | 7.7 | 1.1 |
| Completeness [%] | 83.1 | 66.6 |
| Redundancy | 7.6 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Co-crystals were obtained after 6 days in 20% (w/v) PEG-3350 and 0.2 M DL-malic acid |
