4OVI
Phenylacetone monooxygenase: oxidised enzyme in complex with APADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-23 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 107.238, 107.238, 107.135 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 1.870 |
| R-factor | 0.22382 |
| Rwork | 0.221 |
| R-free | 0.27698 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yls |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.065 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.146 | 0.823 |
| Number of reflections | 59226 | |
| <I/σ(I)> | 0.146 | 1.2 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 10 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | PEG4000 40% w/v, MES 100 mM, NaCl 100 mM |
