4OV2
Crystal structure of C-terminally truncated Neuronal Calcium Sensor (NCS-1) from Rattus norvegicus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979490 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.680, 88.800, 100.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.594 - 2.600 |
R-factor | 0.2226 |
Rwork | 0.220 |
R-free | 0.30820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2YOU |
RMSD bond length | 0.010 |
RMSD bond angle | 1.292 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.594 | 2.770 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 20185 | |
<I/σ(I)> | 2.3 | 1.1 |
Completeness [%] | 98.8 | 98.8 |
Redundancy | 3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 0.2 M sodium chloride, 0.1 M MES, pH 6.0, 16% w/v PEG6000, VAPOR DIFFUSION, SITTING DROP |