4OUO
anti-Bla g 1 scFv
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.500, 51.100, 44.900 |
| Unit cell angles | 90.00, 105.00, 90.00 |
Refinement procedure
| Resolution | 29.654 - 1.800 |
| R-factor | 0.1663 |
| Rwork | 0.165 |
| R-free | 0.19120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ux9 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.358 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 19780 | |
| <I/σ(I)> | 18.1 | 4.5 |
| Completeness [%] | 94.6 | 72 |
| Redundancy | 3.4 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1.8M Ammonium Sulfate, 100mM NaCl, 50mM Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
