4OTP
Crystal structure of the catalytic domain of the human RioK1 atypical protein kinase in complex with ADP/Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 78.760, 78.760, 110.570 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.949 - 2.700 |
R-factor | 0.2077 |
Rwork | 0.204 |
R-free | 0.24340 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.950 | 2.796 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.066 | 0.215 |
Number of reflections | 11336 | |
<I/σ(I)> | 12.69 | 3.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 28% PEG400, 0.1 M HEPES, pH 7.5, 0.2 M calcium chloride, VAPOR DIFFUSION, temperature 293K |