4OSU
Crystal structure of HCMV gB-neutralizing SM5-1 Fab fragment
Experimental procedure
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-14 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.91841 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 92.645, 92.645, 124.646 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.320 - 1.870 |
R-factor | 0.1914 |
Rwork | 0.189 |
R-free | 0.23462 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 2.047 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.320 | 1.920 |
High resolution limit [Å] | 1.870 | 1.870 |
Number of reflections | 43280 | |
Completeness [%] | 99.9 | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 4M sodium formate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |