4OR8

Crystal structure of Marburg virus VP24

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	42.221, 48.078, 64.051
Unit cell angles	88.44, 78.68, 71.34

Refinement procedure

Resolution	39.209 - 2.654
R-factor	0.1834
Rwork	0.180
R-free	0.25490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.616
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1161))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.654
Rmerge	0.060
Number of reflections	13245
<I/σ(I)>	12.77
Completeness [%]	98.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION	6.5	273	0.1M ADA (Hampton Research), 0.1M lithium acetate, 20% glycerol, 2% (v/v) PEG 400 and 8% (v/v) PEG 4000 , pH 6.5, LIQUID DIFFUSION, temperature 273K