4OOR
Ancestral Steroid Receptor 2 DNA binding domain in complex with a steroid response element
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-23 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.472, 80.374, 115.645 |
Unit cell angles | 90.00, 96.74, 90.00 |
Refinement procedure
Resolution | 30.583 - 2.701 |
R-factor | 0.1926 |
Rwork | 0.191 |
R-free | 0.23260 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.766 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_1593) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.760 |
High resolution limit [Å] | 2.700 | 6.650 | 2.700 |
Rmerge | 0.082 | 0.043 | 0.356 |
Number of reflections | 23370 | ||
<I/σ(I)> | 10.5 | ||
Completeness [%] | 97.9 | 99.1 | 82.3 |
Redundancy | 3.5 | 3.6 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293.15 | 20% PEG3350, 50 mM ammonium acetate, 100 mM Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |