Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-05 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.939, 70.621, 75.001 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.338 - 2.004 |
| R-factor | 0.1507 |
| Rwork | 0.148 |
| R-free | 0.19610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q5h |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.077 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: dev_1606)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.340 | 2.076 |
| High resolution limit [Å] | 2.004 | 2.000 |
| Number of reflections | 25874 | |
| Completeness [%] | 99.5 | 98.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 2 M ammonium sulfate, 0.1 M Tris-HCl, 0.02 M Taurine, 5mM Magnesium, VAPOR DIFFUSION, HANGING DROP, pH 9 |
