4ONT
Ternary host recognition complex of complement factor H, C3d, and sialic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-18 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.07 |
| Spacegroup name | P 1 |
| Unit cell lengths | 75.459, 82.536, 85.752 |
| Unit cell angles | 112.50, 110.71, 99.98 |
Refinement procedure
| Resolution | 46.090 - 2.150 |
| R-factor | 0.1749 |
| Rwork | 0.172 |
| R-free | 0.22282 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3oxu |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.527 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.090 | 2.210 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 88718 | |
| <I/σ(I)> | 2.33 | |
| Completeness [%] | 97.2 | 94.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 0.1 M Tris-HCl, pH 9.0, 8% w/v PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
