4ONS
Structural and thermodynamic characterization of cadherin-beta-catenin-alpha-catenin complex formation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-12-16 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 31 |
Unit cell lengths | 96.125, 96.125, 65.517 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.753 - 2.800 |
R-factor | 0.2075 |
Rwork | 0.203 |
R-free | 0.26370 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.576 |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.753 | 38.753 | 2.950 |
High resolution limit [Å] | 2.794 | 8.840 | 2.790 |
Rmerge | 0.028 | 0.496 | |
Number of reflections | 16165 | ||
<I/σ(I)> | 12.4 | 17.6 | 0.3 |
Completeness [%] | 96.2 | 94.9 | 93.1 |
Redundancy | 3.3 | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 283 | 100mM Tris-Cl, 22% polyethylene glycol monomethryl ether, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 283K |