4ONN

Crystal structure of human Mms2/Ubc13 - BAY 11-7082

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	105
Detector technology	CCD
Collection date	2013-09-07
Detector	RAYONIX MX300HE
Wavelength(s)	0.97947
Spacegroup name	P 21 21 21
Unit cell lengths	44.730, 74.533, 91.531
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.766 - 1.500
R-factor	0.1809
Rwork	0.179
R-free	0.21320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.144
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.8.3_1479)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	1.530
High resolution limit [Å]	1.500	4.070	1.500
Rmerge	0.044	0.032	0.560
Number of reflections	47161
<I/σ(I)>	19.8
Completeness [%]	99.9	99.8	99.9
Redundancy	3.5	5.5	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	298	100 mM sodium citrate, 15% PEG 8000, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K