4ONN
Crystal structure of human Mms2/Ubc13 - BAY 11-7082
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2013-09-07 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97947 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.730, 74.533, 91.531 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.766 - 1.500 |
| R-factor | 0.1809 |
| Rwork | 0.179 |
| R-free | 0.21320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.144 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
| Rmerge | 0.044 | 0.032 | 0.560 |
| Number of reflections | 47161 | ||
| <I/σ(I)> | 19.8 | ||
| Completeness [%] | 99.9 | 99.8 | 99.9 |
| Redundancy | 3.5 | 5.5 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | 100 mM sodium citrate, 15% PEG 8000, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
