4ONF
Fab fragment of 3D6 in complex with amyloid beta 1-7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-03-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.810, 69.440, 61.730 |
| Unit cell angles | 90.00, 115.43, 90.00 |
Refinement procedure
| Resolution | 35.097 - 2.000 |
| R-factor | 0.1599 |
| Rwork | 0.158 |
| R-free | 0.20350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ifl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.095 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | COMO |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 59.376 | 59.376 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.065 | 0.042 | 0.230 |
| Total number of observations | 1360 | 8985 | |
| Number of reflections | 32824 | ||
| <I/σ(I)> | 15 | 13.3 | 3.2 |
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 3.8 | 3.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 295 | 30% Peg400, 0.1 M Tris, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
