4ON9
DECH box helicase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2011-07-10 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.999870 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 98.910, 112.990, 124.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.057 - 2.710 |
| R-factor | 0.2209 |
| Rwork | 0.219 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tbk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.907 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 8.020 | 2.870 |
| High resolution limit [Å] | 2.710 | 5.710 | 2.710 |
| Number of reflections | 38281 | ||
| Completeness [%] | 99.0 | 98.9 | 98.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 288 | 1:1 RIG-I SF2 (60mg/ml) : reservoir solution (100mM HEPES pH=6.8, 100mM ammonium sulfate, 6.7%(v/v) Isopropanol, 9%(v/v) PEG4000), 4days, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
