4ON9
DECH box helicase domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2011-07-10 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.999870 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 98.910, 112.990, 124.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.057 - 2.710 |
R-factor | 0.2209 |
Rwork | 0.219 |
R-free | 0.25750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tbk |
RMSD bond length | 0.006 |
RMSD bond angle | 0.907 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 8.020 | 2.870 |
High resolution limit [Å] | 2.710 | 5.710 | 2.710 |
Number of reflections | 38281 | ||
Completeness [%] | 99.0 | 98.9 | 98.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 288 | 1:1 RIG-I SF2 (60mg/ml) : reservoir solution (100mM HEPES pH=6.8, 100mM ammonium sulfate, 6.7%(v/v) Isopropanol, 9%(v/v) PEG4000), 4days, VAPOR DIFFUSION, HANGING DROP, temperature 288K |