3TBK

Mouse RIG-I ATPase Domain

Summary for 3TBK

• Entry DOI: 10.2210/pdb3tbk/pdb
• Descriptor: RIG-I Helicase Domain, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, 1,2-ETHANEDIOL, ... (4 entities in total)
• Functional Keywords: dech helicase, helicase, atp binding, hydrolase
• Biological source: Mus musculus (mouse)
• Cellular location: Cytoplasm : Q6Q899
• Total number of polymer chains: 1
• Total formula weight: 64038.47

Authors

Civril, F.,Bennett, M.D.,Hopfner, K.-P. (deposition date: 2011-08-07, release date: 2011-10-26, Last modification date: 2024-11-20)

Primary citation

Civril, F.,Bennett, M.,Moldt, M.,Deimling, T.,Witte, G.,Schiesser, S.,Carell, T.,Hopfner, K.P.
The RIG-I ATPase domain structure reveals insights into ATP-dependent antiviral signalling.
Embo Rep., 12:1127-1134, 2011

PubMed Abstract: RIG-I detects cytosolic viral dsRNA with 5' triphosphates (5'-ppp-dsRNA), thereby initiating an antiviral innate immune response. Here we report the crystal structure of superfamily 2 (SF2) ATPase domain of RIG-I in complex with a nucleotide analogue. RIG-I SF2 comprises two RecA-like domains 1A and 2A and a helical insertion domain 2B, which together form a 'C'-shaped structure. Domains 1A and 2A are maintained in a 'signal-off' state with an inactive ATP hydrolysis site by an intriguing helical arm. By mutational analysis, we show surface motifs that are critical for dsRNA-stimulated ATPase activity, indicating that dsRNA induces a structural movement that brings domains 1A and 2A/B together to form an active ATPase site. The structure also indicates that the regulatory domain is close to the end of the helical arm, where it is well positioned to recruit 5'-ppp-dsRNA to the SF2 domain. Overall, our results indicate that the activation of RIG-I occurs through an RNA- and ATP-driven structural switch in the SF2 domain.

PubMed: 21979817
DOI: 10.1038/embor.2011.190

Experimental method

X-RAY DIFFRACTION (2.14 Å)