4OML
Crystal structure of the intertwined dimer of the c-Src tyrosine kinase SH3 domain mutant Q128R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-03 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 46.546, 46.546, 128.034 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.220 - 1.600 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jz4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.1.26) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.220 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.073 | 0.654 |
| Number of reflections | 11086 | |
| <I/σ(I)> | 21.4 | 4.4 |
| Completeness [%] | 97.0 | 93.9 |
| Redundancy | 12.9 | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 298 | 0.1M sodium acetate, 1.6 M ammonium sulphate, 10%PEG 300, 10%Glicerol, pH 5.0, vapor diffusion, hanging drop, temperature 298K |
