4OMK
Crystal structure of SPF bound to squalene
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-21 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.270, 73.540, 94.230 |
| Unit cell angles | 90.00, 96.88, 90.00 |
Refinement procedure
| Resolution | 29.887 - 1.750 |
| R-factor | 0.1495 |
| Rwork | 0.148 |
| R-free | 0.17450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o6u |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.706 |
| Data reduction software | MOSFLM |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.052 | 0.317 |
| Number of reflections | 97261 | |
| <I/σ(I)> | 12.7 | 3.6 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 3.1 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 0.2 M Lithium sulfate, 0.1 M Bis-Tris, 25% PEG3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
