4OMA
The crystal structure of methionine gamma-lyase from Citrobacter freundii in complex with L-cycloserine pyridoxal-5'-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-11-11 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.843 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 56.270, 122.890, 126.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.600 |
R-factor | 0.142 |
Rwork | 0.139 |
R-free | 0.19700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rfv |
RMSD bond length | 0.020 |
RMSD bond angle | 1.943 |
Data reduction software | PROCESS |
Data scaling software | PROCESS |
Phasing software | PHASES |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.650 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.048 | 0.396 |
Number of reflections | 55080 | |
<I/σ(I)> | 17.28 | 3.6 |
Completeness [%] | 94.5 | 94.3 |
Redundancy | 4.5 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 303 | 5% PEG2000 MME, 50 mM Tris-HCl, 0.2 mM PLP, 0.25% DTT, pH 8.5, soaked in 50 mM L-cycloserine for 7 minutes, VAPOR DIFFUSION, HANGING DROP, temperature 303K |