4OM5
Crystal structure of CTX A4 from Taiwan Cobra (Naja naja atra)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2009-07-04 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 72.933, 72.933, 249.422 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.050 - 2.550 |
| R-factor | 0.21724 |
| Rwork | 0.215 |
| R-free | 0.26235 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.907 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 5.490 | 2.550 |
| Rmerge | 0.150 | 0.072 | 0.906 |
| Number of reflections | 13697 | ||
| <I/σ(I)> | 6.8 | ||
| Completeness [%] | 100.0 | 99.1 | 100 |
| Redundancy | 41.1 | 35.5 | 41.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 1.3 M ammonium sulfate, 100 mM Tris-HCl, 200 mM lithium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
