4OJK

Structure of the cGMP Dependent Protein Kinase II and Rab11b Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	77
Detector technology	CCD
Collection date	2012-08-08
Detector	RAYONIX MX-225
Wavelength(s)	.97931
Spacegroup name	P 43 21 2
Unit cell lengths	136.007, 136.007, 76.997
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.949 - 2.657
R-factor	0.2037
Rwork	0.201
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f9l
RMSD bond length	0.003
RMSD bond angle	0.694
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.949	2.800
High resolution limit [Å]	2.657	2.660
Rmerge		0.695
Number of reflections	21139
<I/σ(I)>		3.5
Completeness [%]	99.2	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	295	0.056M sodium phosphate monobasic monohydrate, 1.344 M potassium phosphate dibasic, 10 mM ethylenediaminetetraacetic acid disodium salt dehydrate., pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K