4OJC
Crystal structure of the wild-type full-length trimeric ectodomain of the C. elegans fusion protein EFF-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2010-03-28 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.03960 |
Spacegroup name | I 21 3 |
Unit cell lengths | 173.698, 173.698, 173.698 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.420 - 2.930 |
R-factor | 0.2227 |
Rwork | 0.220 |
R-free | 0.27760 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.340 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | BUSTER (2.9.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.090 |
High resolution limit [Å] | 2.930 | 2.930 |
Number of reflections | 18417 | |
<I/σ(I)> | 3.1 | |
Completeness [%] | 97.4 | |
Redundancy | 7.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2% 2-Propanol, 50mM sodium citrate, 0.8-1.2M ammonium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |