4OJC

Crystal structure of the wild-type full-length trimeric ectodomain of the C. elegans fusion protein EFF-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	110
Detector technology	PIXEL
Collection date	2010-03-28
Detector	PSI PILATUS 6M
Wavelength(s)	1.03960
Spacegroup name	I 21 3
Unit cell lengths	173.698, 173.698, 173.698
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.420 - 2.930
R-factor	0.2227
Rwork	0.220
R-free	0.27760
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.340
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHARP
Refinement software	BUSTER (2.9.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.090
High resolution limit [Å]	2.930	2.930
Number of reflections	18417
<I/σ(I)>		3.1
Completeness [%]	97.4
Redundancy	7.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	2% 2-Propanol, 50mM sodium citrate, 0.8-1.2M ammonium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K